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p akt  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p akt
    P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+akt+antibody/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pmc13018931-113-24-27
    Average 99 stars, based on 17210 article reviews
    p akt - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Cyclophilin J inhibited oxidative stress and apoptosis in triple-negative breast cancer cell through activating the PI3K/Akt signaling pathway.
    Article Snippet: Triple-negative breast cancer (TNBC) was a highly aggressive and metastatic subtype of breast cancer characterized by a poor prognosis and limited treatment options.. Clarifying the underlying molecular mechanisms was of significant clinical importance.. In this study, we plotted Kaplan-Meier survival curves based on data from the Human Cancer Database and found that elevated CYPJ expression increased patient mortality risk and decreased survival rates.

    Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation.
    Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494– 1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); AKT antibody (CST, USA, dilution 1:1000, 4691 T); p-AKT antibody (CST, USA, dilution 1:2000, 4060 T). .. Following this, the PVDF membranes were exposed to HRP-conjugated secondary antibodies (Proteintech, China, dilution 1:10,000; SA00001-2) for 2 h

    Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation
    Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494–1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); AKT antibody (CST, USA, dilution 1:1000, 4691 T); p-AKT antibody (CST, USA, dilution 1:2000, 4060 T). .. Following this, the PVDF membranes were exposed to HRP-conjugated secondary antibodies (Proteintech, China, dilution 1:10,000; SA00001-2) for 2 h at ambient temperature.

    Article Title: ATF3 as a molecular nexus linking ferroptosis regulation to sarcopenia pathogenesis via PI3K/Akt pathway activation.
    Article Snippet: After electrophoresis, the proteins were transferred onto a polyvinylidene fluoride membrane (Millipore 05317-10EA, Germany). .. Following this, the membranes were blocked with a rapid blocking solution (Boster AR0041, China) and incubated overnight at 4°C with the following primary antibodies: β-actin antibody (Beyotime AA128, China), ATF3 antibody (Abcam ab254268, UK), GPX4 antibody (Abcam ab125066), xCT antibody (Abcam ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), MAFbx antibody (Abcam, ab168372), and xCT antibody (Abcam, ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), p53 antibody (Santa Cruz Biotechnology sc-126), p21 antibody (Proteintech Group 28248-1-AP, China), Akt antibody (1:1000, Cell Signaling Technology, USA), p-Akt antibody (1:500, Cell Signaling Technology, USA), PI3K antibody (1:1000, Abcam, UK), Jo ur na l P re -p ro of p-PI3K antibody (1:1000, Abcam, UK), GAPDH antibody (Proteintech Group 10494-1-AP).Afterward, the membranes were washed three times with TBST and then incubated with horseradish peroxidase-labeled anti-rabbit (Beyotime A0208) or anti-mouse (Beyotime A0216) secondary antibodies for 60 minutes. .. Following three additional washes with TBST, the enhanced chemiluminescence detection kit (Dingguo ECL-0011) was added, images were captured using a chemiluminescence analysis system (Bio-Rad, USA).

    Article Title: Corneal Sensory Denervation Causes Epithelial Ferroptosis and Delayed Healing in Mice
    Article Snippet: .. Then, the sections were incubated with GPX4 antibodies (DF6701; Affinity Biosciences, Changzhou, China), GSTM1 antibodies (12412-1-AP; Proteintech, Rosemont, IL, USA), p53 antibodies (10442-1-AP; Proteintech), p-AKT antibody (4060; Cell Signaling Technology, Danvers, MA, USA), or p-mTOR antibodies (ab109268; Abcam, Cambridge, UK) overnight at 4°C. .. Following this, the sections were incubated with Invitrogen Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11008; Thermo Fisher Scientific) for 1 hour at room temperature.

    Blocking Assay:

    Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation.
    Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494– 1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); AKT antibody (CST, USA, dilution 1:1000, 4691 T); p-AKT antibody (CST, USA, dilution 1:2000, 4060 T). .. Following this, the PVDF membranes were exposed to HRP-conjugated secondary antibodies (Proteintech, China, dilution 1:10,000; SA00001-2) for 2 h

    Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation
    Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494–1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); AKT antibody (CST, USA, dilution 1:1000, 4691 T); p-AKT antibody (CST, USA, dilution 1:2000, 4060 T). .. Following this, the PVDF membranes were exposed to HRP-conjugated secondary antibodies (Proteintech, China, dilution 1:10,000; SA00001-2) for 2 h at ambient temperature.

    Article Title: ATF3 as a molecular nexus linking ferroptosis regulation to sarcopenia pathogenesis via PI3K/Akt pathway activation.
    Article Snippet: After electrophoresis, the proteins were transferred onto a polyvinylidene fluoride membrane (Millipore 05317-10EA, Germany). .. Following this, the membranes were blocked with a rapid blocking solution (Boster AR0041, China) and incubated overnight at 4°C with the following primary antibodies: β-actin antibody (Beyotime AA128, China), ATF3 antibody (Abcam ab254268, UK), GPX4 antibody (Abcam ab125066), xCT antibody (Abcam ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), MAFbx antibody (Abcam, ab168372), and xCT antibody (Abcam, ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), p53 antibody (Santa Cruz Biotechnology sc-126), p21 antibody (Proteintech Group 28248-1-AP, China), Akt antibody (1:1000, Cell Signaling Technology, USA), p-Akt antibody (1:500, Cell Signaling Technology, USA), PI3K antibody (1:1000, Abcam, UK), Jo ur na l P re -p ro of p-PI3K antibody (1:1000, Abcam, UK), GAPDH antibody (Proteintech Group 10494-1-AP).Afterward, the membranes were washed three times with TBST and then incubated with horseradish peroxidase-labeled anti-rabbit (Beyotime A0208) or anti-mouse (Beyotime A0216) secondary antibodies for 60 minutes. .. Following three additional washes with TBST, the enhanced chemiluminescence detection kit (Dingguo ECL-0011) was added, images were captured using a chemiluminescence analysis system (Bio-Rad, USA).

    Recombinant:

    Article Title: Establishment and Evaluation of HepG2 Cell Insulin Resistance Model
    Article Snippet: HepG2 cells (Wuhan Punosai Life Science and Technology Co., Ltd)., HepG2-specific medium (Wuhan Punosai Life Science and Technology Co., Ltd., Item No. CM-0103), glucosamine hydrochloride (MedChemExpress, Product No. S1635), recombinant human insulin (Shanghai Yuanye Biological Co., Ltd)., sodium palmitate (Xi’an Kunchuang Science and Technology Development Co. .. Ltd)., Sodium Palmitate (Xi’an Kunchuang Science and Technology Development Co., Ltd., product no. SYSJ-KJ002), 2-NBDG (MedChemExpress, product no. HY-116215), Glucose Detection Kit O-toluidine Method (Shanghai Biyuntian Bio-Technology Co., Ltd., product no. S0201M). p-AKT Antibody (Cell Signaling Technology, product no. 4060T), AKT antibody (Cell Signaling Technology, product no. 9272S). β actin (Proteintech Cat No.66009-1-lg), HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Proteintech Cat No. RGAR001). .. HepG2 cells were cultured in HepG2 cell-specific medium (Wuhan Punosai Life Science and Technology Co., Ltd., Item No. CM-0103) at 37°C with 5% CO 2 , and the culture medium was changed every 2~3 days, and the cells were passaged according to their growth.



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