p akt (Cell Signaling Technology Inc)
99
Structured Review
Cell Signaling Technology Inc
p akt
P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+antibody/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pmc13018931-113-24-27
Average 99 stars, based on 17210 article reviews
P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+akt+antibody/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pmc13018931-113-24-27
Average 99 stars, based on 17210 article reviews
p akt - by Bioz Stars,
2026-09
99/100 stars
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Incubation:Article Title: Cyclophilin J inhibited oxidative stress and apoptosis in triple-negative breast cancer cell through activating the PI3K/Akt signaling pathway. Article Snippet: Triple-negative breast cancer (TNBC) was a highly aggressive and metastatic subtype of breast cancer characterized by a poor prognosis and limited treatment options.. Clarifying the underlying molecular mechanisms was of significant clinical importance.. In this study, we plotted Kaplan-Meier survival curves based on data from the Human Cancer Database and found that elevated CYPJ expression increased patient mortality risk and decreased survival rates. Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation. Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494– 1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494–1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); Article Title: ATF3 as a molecular nexus linking ferroptosis regulation to sarcopenia pathogenesis via PI3K/Akt pathway activation. Article Snippet: After electrophoresis, the proteins were transferred onto a polyvinylidene fluoride membrane (Millipore 05317-10EA, Germany). .. Following this, the membranes were blocked with a rapid blocking solution (Boster AR0041, China) and incubated overnight at 4°C with the following primary antibodies: β-actin antibody (Beyotime AA128, China), ATF3 antibody (Abcam ab254268, UK), GPX4 antibody (Abcam ab125066), xCT antibody (Abcam ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), MAFbx antibody (Abcam, ab168372), and xCT antibody (Abcam, ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), p53 antibody (Santa Cruz Biotechnology sc-126), p21 antibody (Proteintech Group 28248-1-AP, China), Article Title: Corneal Sensory Denervation Causes Epithelial Ferroptosis and Delayed Healing in Mice Article Snippet: .. Then, the sections were incubated with GPX4 antibodies (DF6701; Affinity Biosciences, Changzhou, China), GSTM1 antibodies (12412-1-AP; Proteintech, Rosemont, IL, USA), p53 antibodies (10442-1-AP; Proteintech), Blocking Assay:Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation. Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494– 1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); Article Title: NDC80 promotes epithelial to mesenchymal transition of esophageal squamous cell carcinoma through macrophages polarization and PI3K/AKT pathway activation Article Snippet: .. These membranes underwent a blocking step with 5% non-fat dry milk for a duration of 2 h. Incubation with primary antibodies occurred overnight at 4°C: NDC80 antibody (abcam, UK, dilution 1:10,000, ab109496); E-Cadherin antibody (Affinity, USA, dilution 1:1000, AF0131); Vimentin antibody (abcam, UK, dilution 1:3000, ab92547); N-Cadherin antibody (abcam, UK, dilution 1:8000, ab76011); GAPDH antibody (Proteintech, China, dilution 1:10,000, 10,494–1-AP); PI3K antibody (CST, USA, dilution 1:1000, 4257 T); Article Title: ATF3 as a molecular nexus linking ferroptosis regulation to sarcopenia pathogenesis via PI3K/Akt pathway activation. Article Snippet: After electrophoresis, the proteins were transferred onto a polyvinylidene fluoride membrane (Millipore 05317-10EA, Germany). .. Following this, the membranes were blocked with a rapid blocking solution (Boster AR0041, China) and incubated overnight at 4°C with the following primary antibodies: β-actin antibody (Beyotime AA128, China), ATF3 antibody (Abcam ab254268, UK), GPX4 antibody (Abcam ab125066), xCT antibody (Abcam ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), MAFbx antibody (Abcam, ab168372), and xCT antibody (Abcam, ab307601), MuRF1 antibody (Santa Cruz Biotechnology sc-398608, USA), MAFbx antibody (Abcam ab168372), p53 antibody (Santa Cruz Biotechnology sc-126), p21 antibody (Proteintech Group 28248-1-AP, China), Recombinant:Article Title: Establishment and Evaluation of HepG2 Cell Insulin Resistance Model Article Snippet: HepG2 cells (Wuhan Punosai Life Science and Technology Co., Ltd)., HepG2-specific medium (Wuhan Punosai Life Science and Technology Co., Ltd., Item No. CM-0103), glucosamine hydrochloride (MedChemExpress, Product No. S1635), recombinant human insulin (Shanghai Yuanye Biological Co., Ltd)., sodium palmitate (Xi’an Kunchuang Science and Technology Development Co. .. Ltd)., Sodium Palmitate (Xi’an Kunchuang Science and Technology Development Co., Ltd., product no. SYSJ-KJ002), 2-NBDG (MedChemExpress, product no. HY-116215), Glucose Detection Kit O-toluidine Method (Shanghai Biyuntian Bio-Technology Co., Ltd., product no. S0201M). |